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Gaetano Donofrio
Gaetano Donofrio’ s C.V.
Surname: Donofrio Nationality: Italian
First name(s): Gaetano Gender: Male
Academic Title: Associate Professor Date/place of birth: 9-6-65
Saronno (VA), Italy
Addresses
Private: via Nervesa 6, 43100, Parma, Italy
Tel: 00390521221380
E-mail: gaetano.donofrio@unipr.it
Work: Dipartimento di Scienze Medico-Veterinarie, Università di Parma, via del Taglio 10, 43126, Parma, Italy
Tel: 00390521902677 Fax: 00390521902672 E-mail: gaetano.donofrio@unipr.it
Description of Qualifications, Institutions where and Dates on which they were obtained:
First class honour degree in Veterinary Medicine 21-06-91, Parma (Italy)
Ph.D. in Molecular Biotechnology 12-08-95, Perugia and Edinburgh (U.K.), Roslin Institute
Post-doctoral position, 01-01-96, Edinburgh (U.K.), Roslin Institute
Research fellow in the Department of Neuropathology of Zurich University under the supervision of Professor Adriano Aguzzi, on a project concerning Gene-Immunotherapy approach to Prion Disease.
Present Professional Position:
Full Professor in Animal Infectious Disease at the Department of Medical-Veterinary Sciece, University of Parma (Italy).
Previous Professional Posts of Interest or Relevance:
1997: From the first of July to the end of August, short term fellowship at Royal Dick School of Veterinary Medicine, University of Edinburgh (U.K.)
1998: From the first of July to the end of August, short term fellowship at Department of Patho-biology, School of Veterinary Medicine, University of Auburn (U.S.A.)
1999: From the first of July to the end of August, short term fellowship at Department of Patho-biology, School of Veterinary Medicine, University of Auburn (U.S.A.)
2000: From the first of July to the end of August, short term fellowship at Department of Patho-biology, School of Veterinary Medicine, University of Auburn (U.S.A.)
2001-2002: One year Research fellow in the Department of Neuropathology of Zurich University under the supervision of Professor Adriano Aguzzi, on a project concerning Gene-Immunotherapy approach to Prion Disease.
2006: From the first of July to the end of August, short term fellowship at Department of Clinical Science, Royal Veterinary College, University of London (U.K.)
2007: From the first of July to the end of August, short term fellowship at Department of Clinical Science, Royal Veterinary College, University of London (U.K.)
2008: From the first of July to the end of September, as a Wellcome Trust fellowship recipient at Department of Clinical Science, Royal Veterinary College, University of London (U.K.)
2009: From the first of July to the end of August, short term fellowship at Department of Reproductive Immunology, Swansea University (U.K.)
2011: From the 3th of October to the 3th of November, short term fellowship at Vaccine and Gene Therapy Institute, Oregon Primate Research Center, Beaverton, Portland, U.S.A.
Main research interests:
Development and employment of BoHV-4 as a gene delivery vector for vaccinology and oncolysis.
Involvement of BoHV-4 in bovine post-partum metritis.
Viral vector mediated gene transfer for Myeloma cells.
Non-viral gene transfer.
Scientific production
List of papers published on international peer reviewed journals (ISI) provided of an impact factor. 86 papers published on international journals with high impact factor (H index=22).
Papers published:
87) Bovine herpesvirus 4-based vector delivering a hybrid rat/human HER-2 oncoantigen efficiently protects mice from autochthonous Her-2+ mammary cancer.
Jacca S, Rolih V, Quaglino E, Franceschi V, Tebaldi G, Bolli E, Rosamilia A, Ottonello S, Cavallo F, Donofrio G.
Oncoimmunology. 2015 (in press). (impact 6.2)
86) The anti-tumoral effect of lenalidomide is increased in vivo by hypoxia-inducible factor (HIF)-1α inhibition in myeloma cells.
Storti P, Toscani D, Airoldi I, Marchica V, Maiga S, Bolzoni M, Fiorini E, Campanini N, Martella E, Mancini C, Guasco D, Ferri V, Donofrio G, Aversa F, Amiot M, Giuliani N.
Haematologica. 2015 Dec 11. pii: haematol.2015.133736. (impact 5.814)
85) BoHV-4 immediate early 1 gene is a dispensable gene and its product is not a bone marrow stromal cell antigen 2 counteracting factor.
Franceschi V, Capocefalo A, Jacca S, Rosamilia A, Cavirani S, Xu F, Qiao W, Donofrio G.
BMC Vet Res. 2015 Aug 27;11:224. doi: 10.1186/s12917 (impact 1.777)
84) In vivo imaging of the lung inflammatory response to Pseudomonas aeruginosa and its modulation by azithromycin.
Stellari F, Bergamini G, Sandri A, Donofrio G, Sorio C, Ruscitti F, Villetti G, Assael BM, Melotti P, Lleo MM.
J Transl Med. 2015 Aug 4;13:251. doi: 10.1186/s12967-015-0615-9. (impact 3.93)
83) BoHV-4-Based Vector Single Heterologous Antigen Delivery Protects STAT1(-/-) Mice from Monkeypoxvirus Lethal Challenge.
Franceschi V, Parker S, Jacca S, Crump RW, Doronin K, Hembrador E, Pompilio D, Tebaldi G, Estep RD, Wong SW, Buller MR, Donofrio G.
PLoS Negl Trop Dis. 2015 Jun 18;9(6):e0003850. doi: 10.1371/journal.pntd.0003850. eCollection 2015 Jun. (impact 4.4)
82) Cyclodextrin- and calixarene-based polycationic amphiphiles as gene delivery systems: a structure-activity relationship study.
Gallego-Yerga L, Lomazzi M, Franceschi V, Sansone F, Ortiz Mellet C, Donofrio G, Casnati A, García Fernández JM.
Org Biomol Chem. 2015 Feb 14;13(6):1708-23. doi: 10.1039/c4ob02204a. (impact 5.5)
81) Azithromycin inhibits nuclear factor-κB activation during lung inflammation: an in vivo imaging study.
Stellari FF, Sala A, Donofrio G, Ruscitti F, Caruso P, Topini TM, Francis KP, Li X, Carnini C, Civelli M, Villetti G.
Pharmacol Res Perspect. 2014 Oct;2(5):e00058. doi: 10.1002/prp2.58. (in progress)
80) Nonviral Gene-Delivery: Gemini Bispyridinium Surfactant-based DNA Nanoparticles.
Fisicaro E, Compari C, Bacciottini F, Contardi L, Barbero N, Viscardi G, Quagliotto P, Donofrio G, Różycka-Roszak B, Misiak P, Woźniak E, Sansone F.
J Phys Chem B. 2014 Oct 23. (impact 3.377)
79) Interferon gamma mediated BoHV-4 replication restriction in bovine endometrial stromal cells is host IDO1 gene expression independent and BoHV-4 IE2 gene expression dependent.
Jacca S, Franceschi V, Agosti M, Cavirani S, Mistretta F, Donofrio G.
Biology of Reproduction. 2014 Oct 1. pii: biolreprod.114.123000. (impact 4.1)
78) Generation and characterization of the first alpaca cell line suitable for diagnostic and immunization studies.
Franceschi V, Jacca S, Sassu EL, Stellari FF, van Santen VL, G Donofrio.
PLoS One. 2014 Aug 20;9(8):e105643. (impact 3.730)
77) Retinal transduction profiles by high-capacity viral vectors.
Puppo A, Cesi G, Marrocco E, Piccolo P, Jacca S, Shayakhmetov DM, Parks RJ, Davidson BL, Colloca S, Brunetti-Pierri N, Ng P, Donofrio G, Auricchio A.
Gene Ther. 2014 Jul 3. doi: 10.1038/gt.2014.57. (impact 4.321)
76) In vivo Image analysis of BoHV-4-based vector in mice.
Franceschi V, Stellari FF, Mangia C, Jacca S, Lavrentiadou S, Cavirani S, Heikenwalder M, Gaetano Donofrio.
PLoS One. 2014 Apr 21;9(4):e95779. (impact 3.730)
75) Enlightened Mannhemia haemolytica lung inflammation in bovinized mice.
Fabio Franco Stellari, Sophia Lavrentiadou, Francesca Ruscitti, Sarah Jacca, Valentina Franceschi, Maurizio Civelli, Chiara Carnini, Gino Villetti and Gaetano Donofrio.
Veterinary Research 2014 Jan 25;45:8. (impact 3.45)
74) Aurora and IKK kinases cooperatively interact to protect Multiple Myeloma cells from Apo2L/TRAIL.
Mazzera L, Lombardi G, Abeltino M, Ricca M, Donofrio G, Giuliani N, Cantoni A M, Corradi A, Bonati A, and Lunghi P.
Blood 2013 Oct 10;122(15):2641-53. (impact 9.060)
73) Efficient heterologous antigen gene delivery and expression by a replication-attenuated BoHV-4-based vaccine.
Capocefalo A, Mangia C, Franceschi V, Jacca S , van Santen VL, and Donofrio G.
Vaccine 2013 Aug 20;31(37):3906-14. (impact 3.492)
72) Arginine clustering on calix[4]arene macrocycle as novel strategy for improving cell penetration and DNA delivery.
Bagnacani V, Franceschi V, Bassi V, Lomazzi M, Donofrio G*, Sansone F*, Casnati A*, Ungaro R. (*scearing the corresponding author with Sansone and Casnati)
Nature Com. 2013;4:1721. (impact 10.015)
71) Characterization of caprine herpesvirus 1 (CpHV1) glycoprotein E and glycoprotein I ectodomains expressed in mammalian cells.
Bertolotti L, Rosamilia A, Profiti M, Brocchi E, Masoero L, Franceschi V, Tempesta M, Donofrio G, Rosati S.
Veterinary Microbiology 2013 Jun 28;164(3-4):222-8. (impact 3.127)
70) Bovine endometrial stromal cells support tumor necrosis factor alpha induced bovine herpesvirus type 4 enhanced replication.
Sarah Jacca, Valentina Franceschi , Angelo Colagiorgi, Martin Sheldon and Donofrio G.
Biology of Reproduction 2013 May 31;88(5):135. (impact 4.139)
69) Hypoxia-inducible factor (hif)-1α suppression in myeloma cells blocks tumoral growth in vivo inhibiting angiogenesis an bone destruction.
Storti P, Bolzoni M, Donofrio G, Airoldi I, Guasco D, Toscani D, Martella E, Lazzaretti M, Mancini C, Agnelli L, Patrene K, Maïga S, Franceschi V, Colla S, Anderson J, Neri A, Amiot M, Aversa F, Roodman GD, Giuliani N.
Leukemia 2013 Jan 24. (impact 10.164)
68) Clinical protection of goats against CpHV-1 induced genital disease with a BoHV-4-based vector expressing CpHV-1 gD.
Donofrio G, Franceschi V, Lovero A, Capocefalo A, Camero M, Losurdo M, Cavirani S, Marinaro M, Grandolfo E, Buonavoglia C, Tempesta M.
PlosOne 2013;8(1):e52758. (impact 3.730)
67) Bovine herpesvirus 4 glycoprotein B is indispensable for lytic replication irreplaceable by VSVg.
Franceschi V, Capocefalo A, Cavirani S, Donofrio G.
BMC Veterinary Research 2013 Jan 9;9(1):6. (impact 1.861)
66) Myeloma cells inhibit non-canonical Wnt co-recepotr Ror2 expression in human bone marrow osteoprogenitor cells: effect of Wnt5a/Ror2 pathway activation on the osteogenic differentiation impairment induced by myeloma cells.
Bolzoni M, Donofrio G, Storti P, Guasco D, Toscani D, Lazzaretti M, Bonomini S, Agnelli L, Capocefalo A, Palma BD, Neri A, Nicolini F, Lisignoli G, Russo F, Colla S, Aversa F, Giuliani N.
Leukemia. 2013 Feb;27(2):451-63.(impact 10.164)
65) In Vivo Imaging of Transiently Transgenized Mice with a Bovine Interleukin 8 (CXCL8) Promoter/Luciferase Reporter Construct.
Stellari FF, Franceschi V, Capocefalo A, Ronchei M, Facchinetti F, Villetti G, Donofrio G.
Plos One. 2012 June; 7(6):1-9. (impact 3.730)
64) Lower Rim Guanidinocalix[4]arenes: Macrocyclic Nonviral Vectors for Cell Transfection.
Bagnacani V, Franceschi V, Fantuzzi L, Casnati A, *Donofrio G, *Sansone F, Ungaro R.
Bioconjugate Chem. 2012 Apr; 23(5):993–1002 (*shearing the corresponding author with Sansone F.) (impact 4.580)
63) HSV-1-TK armed BoHV-4-based gene delivery vector displays enhanced oncolytic properties in immuno-competent orthotopic syngenic mouse and rat glioma models.
Redaelli M, Franceschi V, Capocefalo A, D'Avella D, Denaro L, Cavirani S, Mucignat-Caretta C, Donofrio G.
Neuro Oncol. 2012 Mar;14(3):288-301. (impact 6.180)
62) Immunization of knock-out α/β interferon receptor mice against lethal bluetongue infection with a BoHV-4-based vector expressing BTV-8 VP2 antigen.
Franceschi V, Capocefalo A, Calvo-Pinilla E, Redaelli M, Mucignat-Caretta C, Mertens P, Ortego J, Donofrio G.
Vaccine. 2011 Apr 5;29(16):3074-82. (impact 3.766)
61) Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U.
Franceschi V, Capocefalo A, Ravanetti L, Vanderplasschen A, Gillet L, Cavirani S, van Santen VL, Donofrio G.
Vet Microbiol. 2011 March 29; 219-231 (impact 3.327)
60) Swine adipose stromal cells loaded with recombinant bovine herpesvirus 4 virions expressing a foreign antigen induce potent humoral immune responses in pigs.
Donofrio G, Taddei S, Franceschi V, Capocefalo A, Cavirani S, Martinelli N, Ottonello S, Ferrari M.
Vaccine. 2011 Jan 29; 867-872 (impact 3.766)
59) HOXB7 expression by myeloma cells regulates their pro-angiogenic properties in multiple myeloma patients.
Storti P, Donofrio G, Colla S, Airoldi I, Bolzoni M, Agnelli L, Abeltino M, Todoerti K, Lazzaretti M, Mancini C, Ribatti D, Bonomini S, Franceschi V, Pistoia V, Lisignoli G, Pedrazzini A, Cavicchi O, Neri A, Rizzoli V, Giuliani N.
Leukemia. 2011 Mar;25(3):527-37. (impact 9.561)
58) Bovine herpesvirus 4 based vector as a potential oncolytic-virus for treatment of glioma.
Redaelli M, Mucignat-Caretta C, Cavaggioni A, Caretta A, D'Avella D, Denaro L, Cavirani S, Donofrio G.
Virol J. 2010 Nov 3;7(1):298. (impact 2.546)
57) Virally and physically transgenized equine adipose-derived stromal cells as a cargo for paracrine secreted factors.
Donofrio G, Capocefalo A, Franceschi V, Morini G, Del Bue M, Conti V, Cavirani S, Grolli S.
BMC Cell Biol. 2010 Sep 23;11:73. (impact 2.464)
56) Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome.
Donofrio G, Capocefalo A, Franceschi V, De Lorenzi L, van Santen V, Parma P.
Virol J. 2010 Sep 21;7:246. (impact 2.546)
55) Low bone marrow oxygen tension and hypoxia-inducible factor-1alpha overexpression characterize patients with multiple myeloma: role on the transcriptional and proangiogenic profiles of CD138(+) cells.
Colla S, Storti P, Donofrio G, Todoerti K, Bolzoni M, Lazzaretti M, Abeltino M, Ippolito L, Neri A, Ribatti D, Rizzoli V, Martella E, Giuliani N.
Leukemia. 2010 Nov;24(11):1967-70. (impact 8.966)
54) The Chemokine IL8 Is Up-Regulated in Bovine Endometrial Stromal Cells by the BoHV-4 IE2 Gene Product, ORF50/Rta: A Step Ahead Toward a Mechanism for BoHV-4 Induced Endometritis.
Donofrio G, Capocefalo A, Franceschi V, Price S, Cavirani S, Sheldon M.
Biology of Reproduction 2010 Dec;83(6):919-28. (impact 3.870)
53) Expression and secretion of Bluetongue virus serotype 8 (BTV-8)VP2 outer capsid protein by mammalian cells.
Capocefalo A, Franceschi V, Mertens PP, Castillo-Olivares J, Cavirani S, Di Lonardo E, Leni Z, Donofrio G.
J Virol Methods. 2010 Nov;169(2):420-4. (impact 2.139)
52) Bovine herpesvirus 4 ORF73 is dispensable for virus growth in vitro, but is essential for virus persistence in vivo.
Thirion M, Machiels B, Farnir F, Donofrio G, Gillet L, Dewals B, Vanderplasschen A.
J Gen Virol. 2010 Oct;91(Pt 10):2574-84. (impact 3.568)
51) Short communication: characterization of a monoclonal antibody for kappa-casein B of cow's milk.
Summer A, Santus E, Casanova L, Joerg H, Rossoni A, Nicoletti C, Donofrio G, Mariani P, Malacarne M.
J Dairy Sci. 2010 Feb;93(2):796-800. (impact 2.497)
50) Isolation and characterization of a strain of Lichtheimia corymbifera (ex Absidia corymbifera) from a case of bovine abortion.
Piancastelli C, Ghidini F, Donofrio G, Jottini S, Taddei S, Cavirani S, Cabassi CS.
Reprod Biol Endocrinol. 2009 Nov 30;7:138. (impact 1.695)
49) Cellular targeting of engineered heterologous antigens is a determinant factor for bovine herpesvirus 4-based vaccine vector development.
Donofrio G, Franceschi V, Capocefalo A, Taddei S, Sartori C, Bonomini S, Cavirani S, Cabassi CS, Flammini CF.
Clin Vaccine Immunol. 2009 Nov;16(11):1675-86. (impact 2.077)
48) Isolation and characterization of bovine herpesvirus 4 (BoHV-4) from a cow affected by post partum metritis and cloning of the genome as a bacterial artificial chromosome.
Donofrio G, Franceschi V, Capocefalo A, Cavirani S, Sheldon IM.
Reprod Biol Endocrinol. 2009 Aug 19;7:83. (impact 2.077)
47) p21(Waf1/Cip1) as a molecular sensor for BoHV-4 replication.
Capocefalo A, Franceschi V, Whitelaw CB, Vasey DB, Lillico SG, Cavirani S, Donofrio G.
J Virol Methods. 2009 Nov;161(2):308-11. Epub 2009 Jun 18. (impact 2.133)
46) Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle.
Sheldon IM, Cronin J, Goetze L, Donofrio G, Schuberth HJ.
Biol Reprod. 2009 Dec;81(6):1025-32. Epub 2009 May 13. (impact 3.300)
45) Bovine endometrial stromal cells display osteogenic properties.
Donofrio G, Franceschi V, Capocefalo A, Cavirani S, Sheldon IM.
Reprod Biol Endocrinol. 2008 Dec 16;6:65. (impact 2.634)
44) Double immunization strategy with a BoHV-4-vectorialized secreted chimeric peptide BVDV-E2/BoHV-1-gD.
Donofrio G, Sartori C, Franceschi V, Capocefalo A, Cavirani S, Taddei S, Flammini CF.
Vaccine. 2008 Nov 11;26(48):6031-42. (impact 3.298)
43) Macrocyclic nonviral vectors: high cell transfection efficiency and low toxicity in a lower rim guanidinium calix[4]arene.
Bagnacani V, Sansone F, Donofrio G, Baldini L, Casnati A, Ungaro R.
Org Lett. 2008 Sep 18;10(18):3953-6. Epub 2008 Aug 23. (impact 5.128)
42) Thermodynamics and biological properties of the aqueous solutions of new glucocationic surfactants.
Fisicaro E, Compari C, Biemmi M, Duce E, Peroni M, Donofrio G, Sansone F, Rózycka-Roszak B, Pruchnik H, Barbero N, Viscardi G, Quagliotto P.
J Phys Chem B. 2008 Aug 7;112(31):9360-70. (impact 4.189)
41) Bacterial infection of endometrial stromal cells influences bovine herpesvirus 4 immediate early gene activation: a new insight into bacterial and viral interaction for uterine disease.
Donofrio G, Ravanetti L, Cavirani S, Herath S, Capocefalo A, Sheldon IM.
Reproduction. 2008 Sep;136(3):361-6. (impact 3.073)
40) Transduction of the rat brain by Bovine Herpesvirus 4.
Redaelli M, Cavaggioni A, Mucignat-Caretta C, Cavirani S, Caretta A, Donofrio G.
Genet Vaccines Ther. 2008 Feb 12;6:6.
39) Assessment of bovine herpesvirus 4 based vector in chicken.
Donofrio G, Manarolla G, Ravanetti L, Sironi G, Cavirani S, Cabassi CS, Flammini CF, Rampin T.
J Virol Methods. 2008 Mar;148(1-2):303-6. (impact 2.077)
38) Establishment of a bovine herpesvirus 4 based vector expressing a secreted form of the bovine viral diarrhoea virus structural glycoprotein E2 for immunization purposes.
Donofrio G, Sartori C, Ravanetti L, Cavirani S, Gillet L, Vanderplasschen A, Taddei S, Flammini CF.
BMC Biotechnol. 2007 Oct 18;7:68. (impact 2.383)
37) Protection against abortion linked to gamma interferon production in pregnant dairy cows naturally infected with Neospora caninum.
López-Gatius F, Almería S, Donofrio G, Nogareda C, García-Ispierto I, Bech-Sàbat G, Santolaria P, Yániz JL, Pabón M, de Sousa NM, Beckers JF.
Theriogenology. 2007 Oct 15;68(7):1067-73. (impact 2.041)
36) The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis.
Colla S, Tagliaferri S, Morandi F, Lunghi P, Donofrio G, Martorana D, Mancini C, Lazzaretti M, Mazzera L, Ravanetti L, Bonomini S, Ferrari L, Miranda C, Ladetto M, Neri TM, Neri A, Greco A, Mangoni M, Bonati A, Rizzoli V, Giuliani N.
Blood. 2007 Dec 15;110(13):4464-75. (impact 10.432)
35) Production of Wnt inhibitors by myeloma cells: potential effects on canonical Wnt pathway in the bone microenvironment.
Giuliani N, Morandi F, Tagliaferri S, Lazzaretti M, Donofrio G, Bonomini S, Sala R, Mangoni M, Rizzoli V.
Cancer Res. 2007 Aug 15;67(16):7665-74. (impact 7.514)
34) Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function.
Donofrio G, Herath S, Sartori C, Cavirani S, Flammini CF, Sheldon IM.
Reproduction. 2007 Jul;134(1):183-97. (impact 3.073)
33) First Serbian isolates of bovine herpesvirus 4 (BoHV-4) from a herd with a history of postpartum metritis.
Nikolin VM, Donofrio G, Milosevic B, Taddei S, Radosavljevic V, Milicevic V.
New Microbiol. 2007 Jan;30(1):53-7. (impact 0.912)
32) Generation of a transposon insertion mutant library for bovine herpesvirus 4 cloned as a bacterial artificial chromosome by in vitro MuA based DNA transposition system.
Donofrio G, Martignani E, Sartori C, Vanderplasschen A, Cavirani S, Flammini CF, Gillet L.
J Virol Methods. 2007 Apr;141(1):63-70. (impact 2.077)
31) DNA condensation and cell transfection properties of guanidinium calixarenes: dependence on macrocycle lipophilicity, size, and conformation.
Sansone F, Dudic M, Donofrio G, Rivetti C, Baldini L, Casnati A, Cellai S, Ungaro R.
J Am Chem Soc. 2006 Nov 15;128(45):14528-36. (impact 8.091)
30) Association between Coxiella burnetii seropositivity and abortion in dairy cattle of Northern Italy.
Cabassi CS, Taddei S, Donofrio G, Ghidini F, Piancastelli C, Flammini CF, Cavirani S.
New Microbiol. 2006 Jul;29(3):211-4. (impact 0.806)
29) Recombinant bovine herpesvirus 4 (BoHV-4) expressing glycoprotein D of BoHV-1 is immunogenic and elicits serum-neutralizing antibodies against BoHV-1 in a rabbit model.
Donofrio G, Cavirani S, Vanderplasschen A, Gillet L, Flammini CF.
Clin Vaccine Immunol. 2006 Nov;13(11):1246-54. (impact 2.598)
28) Expression of bovine viral diarrhea virus glycoprotein E2 as a soluble secreted form in a Mammalian cell line.
Donofrio G, Bottarelli E, Sandro C, Flammini CF.
Clin Vaccine Immunol. 2006 Jun;13(6):698-701. (impact 2.598)
27) Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo.
Donofrio G, Martignani E, Poli E, Lange C, Martini FM, Cavirani S, Cabassi CS, Taddei S, Flammini CF.
J Virol Methods. 2006 Sep;136(1-2):126-36 . (impact 2.096)
26) Outcome of bovine herpesvirus 4 infection following direct viral injection in the lateral ventricle of the mouse brain.
Donofrio G, Cavaggioni A, Bondi M, Cavirani S, Flammini CF, Mucignat-Caretta C.
Microbes Infect. 2006 Mar;8(3):898-904. (impact 3.127)
25) Association between Neospora caninum antibodies and blue tongue vaccination in dairy cows.
Cavirani S, Cabassi CS, Taddei S, Donofrio G, Bottarelli E.
Vet Res Commun. 2005 Aug;29 Suppl 2:233-6. (impact 0.328)
24) Establishment, differentiation, electroporation, viral transduction, and nuclear transfer of bovine and porcine mesenchymal stem cells.
Colleoni S, Donofrio G, Lagutina I, Duchi R, Galli C, Lazzari G.
Cloning Stem Cells. 2005;7(3):154-66. (impact 2.744)
23) Potential secondary pathogenic role for bovine herpesvirus 4.
Donofrio G, Cavirani S, van Santen V, Flammini CF.
J Clin Microbiol. 2005 Jul;43(7):3421-6.
22) Paracrine inhibition of prion propagation by anti-PrP single-chain Fv miniantibodies.
Donofrio G, Heppner FL, Polymenidou M, Musahl C, Aguzzi A.
J Virol. 2005 Jul;79(13):8330-8.
21) Exploiting persistent infection for selection of bovine herpesvirus 4 recombinants.
Donofrio G, Martignani E, Cavirani S, Flammini CF.
J Virol Methods. 2005 Sep;128(1-2):6-13.
20) Susceptibility of bovine mesenchymal stem cells to bovine herpesvirus 4.
Donofrio G, Colleoni S, Galli C, Lazzari G, Cavirani S, Flammini CF.
J Virol Methods. 2005 Aug;127(2):168-70.
19) Development of bovine herpesvirus 4 as an expression vector using bacterial artificial chromosome cloning.
Gillet L, Daix V, Donofrio G, Wagner M, Koszinowski UH, China B, Ackermann M, Markine-Goriaynoff N, Vanderplasschen A.
J Gen Virol. 2005 Apr;86(Pt 4):907-17.
18) Biologically active bisquaternary ammonium chlorides: physico-chemical properties of long chain amphiphiles and their evaluation as non-viral vectors for gene delivery.
Fisicaro E, Compari C, Duce E, Donofrio G, Rózycka-Roszak B, Woźniak E.
Biochim Biophys Acta. 2005 Mar 11;1722(2):224-33.
17) A general synthesis of water soluble upper rim calix[n]arene guanidinium derivatives which bind to plasmid DNA
Dudic M, Colombo A, Sansone F, Casnati A, Donofrio G, Ungano R.
Tetrahedron. 2004 Dec 6; 60(50):11613-11618.
16) Bovine herpesvirus 4 infects differentiated neuronal cells in culture and establish persistent infection upon selection.
Donofrio G, Grandi G, Cavirani S, Taddei S, Flammini C.
J Neurovirol. 2004 Apr;10(2):123-30.
15) Activation of bovine herpesvirus 4 lytic replication in a non-permissive cell line by overexpression of BoHV-4 immediate early (IE) 2 gene.
Donofrio G, Cavirani S, Taddei S, Flammini CF.
J Virol Methods. 2004 Mar 15;116(2):203-7.
14) Interaction of a green recombinant bovine herpesvirus 4 with in vitro-produced bovine embryos.
Donofrio G, Galli C, Lazzari G, van Santen VL, Cavirani S, Flammini CF.
Vet Res Commun. 2003 Jul;27(5):415-24.
13) Isolation of Bartonella henselae from domestic cats in an Italian urban area.
Cabassi CS, Farnetti E, Casali B, Taddei S, Donofrio G, Galvani G, Cavirani S.
New Microbiol. 2002 Apr;25(2):253-7.
12) Potential of bovine herpesvirus 4 as a gene delivery vector.
Donofrio G, Cavirani S, Simone T, van Santen VL.
J Virol Methods. 2002 Mar;101(1-2):49-61.
11) Association between Chlamydia psittaci seropositivity and abortion in Italian dairy cows.
Cavirani S, Cabassi CS, Donofrio G, De Iaco B, Taddei S, Flammini CF.
Prev Vet Med. 2001 Jul 19;50(1-2):145-51.
10) A bovine macrophage cell line supports bovine herpesvirus-4 persistent infection.
Donofrio G, van Santen VL.
J Gen Virol. 2001 May;82(Pt 5):1181-5.
9) Detection of bovine herpesvirus 4 (BoHV-4) DNA in the cell fraction of milk of dairy cattle with history of BoHV-4 infection.
Donofrio G, Flammini CF, Scatozza F, Cavirani S.
J Clin Microbiol. 2000 Dec;38(12):4668-71.
8) Molecular typing of a BHV-4 (bovine herpesvirus 4) field isolate.
Donofrio G, Cavirani S, Flammini CF, Scatozza F.
Vet Res Commun. 2000 Sep;24(6):411-22.
7) Transfection of bovine cell culture with bovine herpesvirus 4 DNA obtained by cell nuclear extraction.
Donofrio G, Cavirani S, Whitelaw B, Flammini CF, Scatozza F.
New Microbiol. 2000 Apr;23(2):129-35.
6) Establishment of a cell line persistently infected with bovine herpesvirus-4 by use of a recombinant virus.
Donofrio G, Cavirani S, van Santen VL.
J Gen Virol. 2000 Jul;81(Pt 7):1807-14.
5) Matrix attachment region regulates basal beta-lactoglobulin transgene expression.
Whitelaw CB, Grolli S, Accornero P, Donofrio G, Farini E, Webster J.
Gene. 2000 Feb 22;244(1-2):73-80.
4) Seroprevalence to bovine immunodeficiency virus and lack of association with leukocyte counts in Italian dairy cattle.
Cavirani S, Donofrio G, Chiocco D, Foni E, Martelli P, Allegri G, Cabassi CS, De Iaco B, Flammini CF.
Prev Vet Med. 1998 Dec 1;37(1-4):147-57.
3) Expression of c-myc is down-regulated as mouse mammary epithelial cells become confluent.
Grolli S, Accornero P, Ramoni R, Donofrio G, Whitelaw CB.
Biochem Biophys Res Commun. 1997 Oct 20;239(2):566-9.
2) Intronic sequences modulate the sensitivity of beta-lactoglobulin transgenes to position effects.
Webster J, Donofrio G, Wallace R, Clark AJ, Whitelaw CB.
Gene. 1997 Jul 9;193(2):239-43.
1) Comparable processing of beta-lactoglobulin pre-mRNA in cell culture and transgenic mouse models.
Donofrio G, Bignetti E, Clark AJ, Whitelaw CB.
Mol Gen Genet. 1996 Sep 25;252(4):465-9.