Current research activity is focused on functional genomics of bacteria in relation to infectious disease. This is particularly aimed at diseases caused by Streptococci. With strategic aims of identification of genes associated with disease and their functional role in vivo and the consequence of allelic variation in the bacterial population. To aid with the initial stages (gene identification) we have developed a simple and rapid genome wide mutation mapping system that produces similar outputs to Tradis and Tnseq. The output is of value to the pharmaceutical industry in the identification of sub-units for vaccines, gene targets for rational attenuation. For academia, it identifies networks of genes (products) for further definition of their role and mechanistic analysis in vitro.